REDUCTION OF TETRAZOLIUM SALTS BY PLEUROPNEUMONIALIKE ORGANISMS
نویسندگان
چکیده
منابع مشابه
Reduction of tetrazolium salts by pleuropneumonialike organisms.
Tetrazolium salts have proved useful as indicators of electron transfer. Compounds such as triphenyltetrazolium chloride' are more practical than indicators like methylene blue since they are converted from a soluble colorless salt to an insoluble formazan in the reduced state. They also provide similar convenience in detecting enzymatic activity and specific sites of such activity (Bielig, Kau...
متن کاملT-Form colonies of pleuropneumonialike organisms.
During the course of previous studies on pleuropneuimonialike organisms (PPLO) and their possible role in the etiology of nongonococcal urethritis (NGU), a minute, morphologically distinct PPLO colony form was observed (Shepard, 1954.) More recent studies have substantiated this observation. These minute colonies of pleuropneumonialike organisms were designated T-form (tiny) PPLO colonies. They...
متن کاملAmino acid metabolism by pleuropneumonialike organisms. II. Glutamine.
A previous examination of the over-all catabolic activity of certain strains of pleuropneumonialike organisms of human origin toward amino acids demonstrated that only glutamine and arginine were readily degraded (Smith, 1955a). L-Glutamine was completely converted to glutamic acid by some mechanism of deamidation. Levintow and Meister (1954) reported on the ability of a purified pea enzyme, ca...
متن کاملQuantitative measurement of bacterial growth by the reduction of tetrazolium salts.
Tetrazolium salts can be used for measuring the reducing activity of microbial cultures colorimetrically, because the reduced form of these redox indicators is conspicuously colored (B. Jambor, Tetrazolium Salze in der Biologie, G. Fischer, Jena, 1960; B. J. Liska et al., J. Dairy Sci. 41:776, 1958). In an actively growing culture, the reducing activity is proportional with the number of bacter...
متن کاملAcceleration of tetrazolium reduction by bacteria.
Conditions were assessed which would permit more rapid recognition of bacterial growth than has been previously reported using tetrazolium salts. Microtitration trays were used. 2-(p-Iodophenyl)-3(p-nitrophenyl)-5-phenyltetrazolium chloride is rapidly reduced by respiring cells in tissue homogenates but is more toxic than other tetrazoliums when added to growing bacterial cultures. Phenazine me...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1953
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.65.3.245-251.1953